dc.contributor	Meneses Ramírez, Erick Alejandro
dc.contributor.author	Arango Cortés, Juan Daniel
dc.contributor.author	Henao Álvarez, Jéssica
dc.contributor.author	Montoya Restrepo, Maria Camila
dc.contributor.author	Salcedo Benavides, Gloria Emilce
dc.date.accessioned	2020-10-15T22:15:38Z
dc.date.available	2020-10-15T22:15:38Z
dc.date.copyright	2020-09-29
dc.date.issued	2020-09-29
dc.identifier.uri	http://hdl.handle.net/10946/4879
dc.description.abstract	The discoveries and advances around molecular biology and its interrelation with other sciences or disciplines have allowed the development of techniques for the modification of genetic information. Recombinant protein expression allows us to extract genetic material from a native cell and insert it into a different cell line in order to obtain a target peptide easily and quickly. Prokaryotic platforms, such as E. coli, are the most widely used systems thanks to the advantages it offers for the execution of the process. However, obtaining adequate protein yield through an efficient process requires the optimization of various parameters that influence expression results. For this reason, during the last decade, statistical techniques like Design of Experiment (DoE) have been applied. Some of them, such as the Response Surface Methodology (RSM), allow the optimization, evaluation and validation of these parameters in order to obtain proteins with high performance, quality and efficiency.	spa
dc.description.sponsorship	Instituto Colombiano de Medicina Tropical (ICMT)	spa
dc.language.iso	en	spa
dc.subject	Recombinant proteins	spa
dc.subject	Expression optimization	spa
dc.subject	Design of experiments	spa
dc.subject	Response surface methodology	spa
dc.title	A Method to Optimize Expression of Recombinant Proteins in E. coli System Using Design of Experiments Techniques	spa
dc.type	Trabajo de Grado	spa
dc.rights.license	Restringido	spa
dc.contributor.role	Asesor	spa

A Method to Optimize Expression of Recombinant Proteins in E. coli System Using Design of Experiments Techniques

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